› 1pk8

X-ray diffraction
2.1Å resolution

Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP

Released:
DOI: 10.2210/pdb1pk8/pdb
PDB entry 1pk8 coloured by chain, front view.
PDB entry 1pk8 coloured by chain, side view.
PDB entry 1pk8 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Tetramerization and ATP binding by a protein comprising the A, B, and C domains of rat synapsin I.
Brautigam CA, Chelliah Y, Deisenhofer J
J Biol Chem 279 11948-56 (2004)
PMID: 14688264

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synapsin-1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 422 amino acids
Theoretical weight: 45.54 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09951 (Residues: 2-421; Coverage: 60%)
Gene name: Syn1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 8 x CA
Ligand ATP 8 x ATP
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 70.6Å b: 78.4Å c: 135Å
α: 80.6° β: 76.9° γ: 71.8°
R-values:
R R work R free
0.235 0.222 0.259
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Synapsin Isoforms and Synaptic Vesicle Trafficking.
Song et al. (2015)
1 more

5 mentions without citation

Dynamin is primed at endocytic sites for ultrafast endocytosis.
Imoto et al. (2022)
4 more