1pjt

X-ray diffraction
2.8Å resolution

The structure of the Ser128Ala point-mutant variant of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis

Released:

Function and Biology Details

Reactions catalysed:
Precorrin-2 + NAD(+) = sirohydrochlorin + NADH
(1a) S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1
Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150613 (preferred)
Entry contents:
1 distinct polypeptide molecule

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 59.539Å b: 120.319Å c: 130.036Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.226 0.291
Expression system: Escherichia coli