› 1pew

X-ray diffraction
1.6Å resolution

High Resolution Crystal Structure of Jto2, a mutant of the non-amyloidogenic Lamba6 Light Chain, Jto

Released:
DOI: 10.2210/pdb1pew/pdb
PDB entry 1pew coloured by chain, front view.
PDB entry 1pew coloured by chain, side view.
PDB entry 1pew coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of light chain amyloidogenicity: comparison of the thermodynamic properties, fibrillogenic potential and tertiary structural features of four Vlambda6 proteins.
Wall JS, Gupta V, Wilkerson M, Schell M, Loris R, Adams P, Solomon A, Stevens F, Dealwis C
J Mol Recognit 17 323-31
PMID: 15227639

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin lambda variable 6-57 Chains: A, B
Molecule details ›
Chains: A, B
Length: 109 amino acids
Theoretical weight: 11.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01721 (Residues: 20-117; Coverage: 98%)
Gene name: IGLV6-57
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand CD 4 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P4122
Unit cell:
a: 73.577Å b: 73.577Å c: 92.298Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.295 0.252 0.273
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

How I treat amyloidosis.
Comenzo RL. (2009)
8 more

2 mentions without citation

Systemic amyloidoses.
Blancas-Mejía et al. (2013)
1 more