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X-ray diffraction
2.15Å resolution

THE 2.15 A CRYSTAL STRUCTURE OF A TRIPLE MUTANT PLASTOCYANIN FROM THE CYANOBACTERIUM SYNECHOCYSTIS SP. PCC 6803

Released:
DOI: 10.2210/pdb1pcs/pdb
PDB entry 1pcs coloured by chain, front view.
PDB entry 1pcs coloured by chain, side view.
PDB entry 1pcs coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
The 2.15 A crystal structure of a triple mutant plastocyanin from the cyanobacterium Synechocystis sp. PCC 6803.
Romero A, De la Cerda B, Varela PF, Navarro JA, Hervás M, De la Rosa MA
J Mol Biol 275 327-36 (1998)
PMID: 9466912

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plastocyanin Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 10.33 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P21697 (Residues: 29-126; Coverage: 100%)
Gene names: petE, sll0199
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P3221
Unit cell:
a: 34.3Å b: 34.3Å c: 111.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.167 0.22
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Paramagnetic resonance of biological metal centers.
Ubbink et al. (2002)
9 other articles

3 mentions without citation

HullRad: Fast Calculations of Folded and Disordered Protein and Nucleic Acid Hydrodynamic Properties.
Fleming et al. (2018)
2 more