› 1p4e

X-ray diffraction
2.7Å resolution

Flpe W330F mutant-DNA Holliday Junction Complex

Released:
DOI: 10.2210/pdb1p4e/pdb
PDB entry 1p4e coloured by chain, front view.
PDB entry 1p4e coloured by chain, side view.
PDB entry 1p4e coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The role of the conserved Trp330 in Flp-mediated recombination. Functional and structural analysis.
Chen Y, Rice PA
J Biol Chem 278 24800-7 (2003)
PMID: 12716882

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114355 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
Site-specific recombinase Flp Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 49.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03870 (Residues: 3-423; Coverage: 100%)
Gene names: FLP1, R0010W
Sequence domains:
Structure domains:
Site-specific recombinase Flp Chains: C, D
Molecule details ›
Chains: C, D
Length: 429 amino acids
Theoretical weight: 49.46 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03870 (Residues: 3-423; Coverage: 100%)
Gene names: FLP1, R0010W
Sequence domains:
Structure domains:
5'-D(*TP*AP*AP*GP*TP*TP*CP*CP*TP*AP*TP*TP*C)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 13 nucleotides
Theoretical weight: 3.92 KDa
5'-D(*TP*TP*TP*AP*AP*AP*AP*GP*AP*AP*TP*AP*GP*GP*AP*AP*CP*TP*TP*C)-3' Chains: I, J
Molecule details ›
Chains: I, J
Length: 20 nucleotides
Theoretical weight: 6.17 KDa
33-MER Chains: G, H
Molecule details ›
Chains: G, H
Length: 33 nucleotides
Theoretical weight: 10.13 KDa

Ligands and Environments

1 bound ligand:
Ligand 2PO 1 x 2PO
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 79.76Å b: 116.715Å c: 142.053Å
α: 90° β: 97.64° γ: 90°
R-values:
R R work R free
0.236 0.236 0.275
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mechanisms of site-specific recombination.
Grindley et al. (2006)
3 more

1 mention without citation

A DNA structural alphabet provides new insight into DNA flexibility.
Schneider et al. (2018)