› 1opx

X-ray diffraction
2.8Å resolution

Crystal structure of the traffic ATPase (HP0525) of the Helicobacter pylori type IV secretion system bound by sulfate

Released:
DOI: 10.2210/pdb1opx/pdb
PDB entry 1opx coloured by chain, front view.
PDB entry 1opx coloured by chain, side view.
PDB entry 1opx coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion.
Savvides SN, Yeo HJ, Beck MR, Blaesing F, Lurz R, Lanka E, Buhrdorf R, Fischer W, Haas R, Waksman G
EMBO J 22 1969-80 (2003)
PMID: 12727865

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer (preferred)
homo dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-181366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial type II secretion system protein E domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.91 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BK04 (Residues: 1-330; Coverage: 100%)
Gene names: ACM37_01770, C2R45_06065, C2R60_04995, C2R64_07520, C2R66_03525, C2R72_03110, C2S01_07015, C2S26_06255, CV728_03100, D8X81_02825, EC515_06990, EC547_07075, EC572_06310, EC594_00565, EDB76_02000, F7219_04320, F7220_05215, HP0525, SE88_02715, cag-alfa, cag-alpha, cagAlpha, cagalpha, virB11
Sequence domains: Type II/IV secretion system protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand 2PE 1 x 2PE
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6322
Unit cell:
a: 111.28Å b: 111.28Å c: 230.24Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.25 0.324
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Mobile genetic elements: the agents of open source evolution.
Frost et al. (2005)
50 more

1 mention without citation

Multiple conformations facilitate PilT function in the type IV pilus.
McCallum et al. (2019)