› 1oow

X-ray diffraction
2Å resolution

The crystal structure of the spinach plastocyanin double mutant G8D/L12E gives insight into its low reactivity towards photosystem 1 and cytochrome f

Released:
DOI: 10.2210/pdb1oow/pdb
PDB entry 1oow coloured by chain, front view.
PDB entry 1oow coloured by chain, side view.
PDB entry 1oow coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
The crystal structure of the spinach plastocyanin double mutant G8D/L12E gives insight into its low reactivity towards photosystem 1 and cytochrome f.
Jansson H, Okvist M, Jacobson F, Ejdebäck M, Hansson O, Sjölin L
Biochim Biophys Acta 1607 203-10 (2003)
PMID: 14670610

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plastocyanin, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 10.49 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli
UniProt:
  • Canonical: P00289 (Residues: 70-168; Coverage: 59%)
Gene name: PETE
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P3121
Unit cell:
a: 74.761Å b: 74.761Å c: 31.383Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.183 0.232
Expression system: Escherichia coli

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Citations

2 review citations

Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl).
Hänsch et al. (2009)
1 more