› 1ojg

Solution NMR

Sensory domain of the membraneous two-component fumarate sensor DcuS of E. coli

Released:
DOI: 10.2210/pdb1ojg/pdb
PDB entry 1ojg coloured by chain, ensemble of 10 models, front view.
PDB entry 1ojg coloured by chain, ensemble of 10 models, side view.
PDB entry 1ojg coloured by chain, ensemble of 10 models, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
The NMR structure of the sensory domain of the membranous two-component fumarate sensor (histidine protein kinase) DcuS of Escherichia coli.
Pappalardo L, Janausch IG, Vijayan V, Zientz E, Junker J, Peti W, Zweckstetter M, Unden G, Griesinger C
J Biol Chem 278 39185-8 (2003)
PMID: 12907689

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor histidine kinase DcuS Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 15.1 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEC8 (Residues: 45-180; Coverage: 25%)
Gene names: JW4086, b4125, dcuS, yjdH
Sequence domains: Single cache domain 3
Structure domains: PAS domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: RESTRAINED MOLECULAR DYNAMICS
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Stimulus perception in bacterial signal-transducing histidine kinases.
Mascher et al. (2006)
14 more

6 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
5 more