› 1ofq

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF THE TYROSINE-REGULATED 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH MANGANESE(II)

Released:
DOI: 10.2210/pdb1ofq/pdb
PDB entry 1ofq coloured by chain, front view.
PDB entry 1ofq coloured by chain, side view.
PDB entry 1ofq coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Substrate and metal complexes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae provide new insights into the catalytic mechanism.
König V, Pfeil A, Braus GH, Schneider TR
J Mol Biol 337 675-90 (2004)
PMID: 15019786

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 370 amino acids
Theoretical weight: 39.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32449 (Residues: 1-370; Coverage: 100%)
Gene names: ARO4, YBR1701, YBR249C
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P3121
Unit cell:
a: 105.731Å b: 105.731Å c: 268.782Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.245
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 review citations

The diversity of allosteric controls at the gateway to aromatic amino acid biosynthesis.
Light et al. (2013)
1 more