› 1oa3

X-ray diffraction
1.7Å resolution

Comparison of Family 12 Glycoside Hydrolases and Recruited Substitutions Important for Thermal Stability

Released:
DOI: 10.2210/pdb1oa3/pdb
PDB entry 1oa3 coloured by chain, front view.
PDB entry 1oa3 coloured by chain, side view.
PDB entry 1oa3 coloured by chain, top view.
Source organism: Trichoderma citrinoviride
Primary publication:
Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability.
Sandgren M, Gualfetti PJ, Shaw A, Gross LS, Saldajeno M, Day AG, Jones TA, Mitchinson C
Protein Sci 12 848-60 (2003)
PMID: 12649442

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 218 amino acids
Theoretical weight: 23.49 KDa
Source organism: Trichoderma citrinoviride
Expression system: Aspergillus niger
UniProt:
  • Canonical: Q8NJY6 (Residues: 17-234; Coverage: 100%)
Gene name: cel12A
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 62.49Å b: 77.547Å c: 83.41Å
α: 90° β: 98.46° γ: 90°
R-values:
R R work R free
0.193 0.192 0.217
Expression system: Aspergillus niger

Quick links

Citations

6 review citations

Directed evolution of industrial enzymes: an update.
Cherry et al. (2003)
5 more

4 mentions without citation

Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability.
Sandgren et al. (2003)
3 more