› 1o9j

X-ray diffraction
2.4Å resolution

The X-ray crystal structure of eta-crystallin

Released:
DOI: 10.2210/pdb1o9j/pdb
PDB entry 1o9j coloured by chain, front view.
PDB entry 1o9j coloured by chain, side view.
PDB entry 1o9j coloured by chain, top view.
Source organism: Elephantulus edwardii
Primary publication:
Crystal structure of eta-crystallin: adaptation of a class 1 aldehyde dehydrogenase for a new role in the eye lens.
Bateman OA, Purkiss AG, van Montfort R, Slingsby C, Graham C, Wistow G
Biochemistry 42 4349-56 (2003)
PMID: 12693930

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, cytosolic 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 501 amino acids
Theoretical weight: 54.6 KDa
Source organism: Elephantulus edwardii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q28399 (Residues: 1-501; Coverage: 100%)
Gene name: ALDH1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand DTT 1 x DTT
Ligand DTU 1 x DTU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 80.869Å b: 136.407Å c: 84.988Å
α: 90° β: 102.62° γ: 90°
R-values:
R R work R free
0.184 0.18 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Single-gene disorders: what role could moonlighting enzymes play?
Sriram et al. (2005)
3 more

1 mention without citation

Adenine binding mode is a key factor in triggering the early release of NADH in coenzyme A-dependent methylmalonate semialdehyde dehydrogenase.
Bchini et al. (2012)