1o6k

X-ray diffraction
1.7Å resolution

Structure of activated form of PKB kinase domain S474D with GSK3 peptide and AMP-PNP

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-152088 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RAC-beta serine/threonine-protein kinase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 39.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P31751 (Residues: 146-481; Coverage: 70%)
Gene name: AKT2
Sequence domains:
Structure domains:
Glycogen synthase kinase-3 beta Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P49841 (Residues: 3-12; Coverage: 2%)
Gene name: GSK3B

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 44.906Å b: 60.998Å c: 129.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.234
Expression system: Spodoptera frugiperda