1nx8

X-ray diffraction
2.3Å resolution

Structure of carbapenem synthase (CarC) complexed with N-acetyl proline

Released:
Source organism: Pectobacterium carotovorum
Primary publication:
Crystal structure of carbapenem synthase (CarC).
J Biol Chem 278 20843-50 (2003)
PMID: 12611886

Function and Biology Details

Reaction catalysed:
(3S,5S)-carbapenam-3-carboxylate + 2-oxoglutarate + O(2) = (5R)-carbapen-2-em-3-carboxylate + succinate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
PDBe Complex ID:
PDB-CPX-194980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(5R)-carbapenem-3-carboxylate synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 273 amino acids
Theoretical weight: 31.65 KDa
Source organism: Pectobacterium carotovorum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XB59 (Residues: 1-273; Coverage: 100%)
Gene name: carC
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: C2221
Unit cell:
a: 80.443Å b: 164.082Å c: 146.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.275
Expression system: Escherichia coli BL21(DE3)