› 1nui

X-ray diffraction
2.9Å resolution

Crystal Structure of the primase fragment of Bacteriophage T7 primase-helicase protein

Released:
DOI: 10.2210/pdb1nui/pdb
PDB entry 1nui coloured by chain, front view.
PDB entry 1nui coloured by chain, side view.
PDB entry 1nui coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Modular architecture of the bacteriophage T7 primase couples RNA primer synthesis to DNA synthesis.
Kato M, Ito T, Wagner G, Richardson CC, Ellenberger T
Mol Cell 11 1349-60 (2003)
PMID: 12769857

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA helicase/primase Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.4 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03692 (Residues: 1-255; Coverage: 45%)
Gene name: 4
Sequence domains:
Structure domains: N-terminal domain of TfIIb

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P3121
Unit cell:
a: 136.459Å b: 136.46Å c: 85.445Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.235 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Structure, molecular mechanisms, and evolutionary relationships in DNA topoisomerases.
Corbett et al. (2004)
10 more

21 mentions without citation

Mechanism and evolution of DNA primases.
Kuchta et al. (2010)
20 more