› 1nph

X-ray diffraction
3Å resolution

Gelsolin Domains 4-6 in Active, Actin Free Conformation Identifies Sites of Regulatory Calcium Ions

Released:
DOI: 10.2210/pdb1nph/pdb
PDB entry 1nph coloured by chain, front view.
PDB entry 1nph coloured by chain, side view.
PDB entry 1nph coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Gelsolin domains 4-6 in active, actin-free conformation identifies sites of regulatory calcium ions.
Kolappan S, Gooch JT, Weeds AG, McLaughlin PJ
J Mol Biol 329 85-92 (2003)
PMID: 12742020

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gelsolin Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 36.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13020 (Residues: 439-767; Coverage: 44%)
Gene names: Gsb, Gsn
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P41212
Unit cell:
a: 122.52Å b: 122.52Å c: 82.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The gelsolin family of actin regulatory proteins: modular structures, versatile functions.
McGough et al. (2003)
2 more

3 mentions without citation

Visualizing Temperature Mediated Activation of Gelsolin and Its Deactivation By Pip2: A Saxs Based Study.
Badmalia et al. (2017)
2 more