› 1nn8

Electron Microscopy
15Å resolution

CryoEM structure of poliovirus receptor bound to poliovirus

Released:
DOI: 10.2210/pdb1nn8/pdb
PDB entry 1nn8 coloured by chain, front view.
PDB entry 1nn8 coloured by chain, side view.
PDB entry 1nn8 coloured by chain, top view.
Source organisms:
Primary publication:
Complexes of poliovirus serotypes with their common cellular receptor, CD155.
He Y, Mueller S, Chipman PR, Bator CM, Peng X, Bowman VD, Mukhopadhyay S, Wimmer E, Kuhn RJ, Rossmann MG
J Virol 77 4827-35 (2003)
PMID: 12663789

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 420-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136701 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Poliovirus receptor Chains: R, S, T
Molecule details ›
Chains: R, S, T
Length: 302 amino acids
Theoretical weight: 32.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P15151 (Residues: 28-329; Coverage: 76%)
Gene names: PVR, PVS
Sequence domains:
Capsid protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 302 amino acids
Theoretical weight: 33.33 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Homo sapiens
UniProt:
  • Canonical: P03300 (Residues: 580-881; Coverage: 14%)
Sequence domains: picornavirus capsid protein
Capsid protein VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 272 amino acids
Theoretical weight: 30.08 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Homo sapiens
UniProt:
  • Canonical: P03300 (Residues: 70-341; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Capsid protein VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 235 amino acids
Theoretical weight: 26.24 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Homo sapiens
UniProt:
  • Canonical: P03300 (Residues: 342-576; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 68 amino acids
Theoretical weight: 7.39 KDa
Source organism: Human poliovirus 1 Mahoney
Expression system: Homo sapiens
UniProt:
  • Canonical: P03300 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)

Ligands and Environments

1 bound ligand:
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 15Å
Expression system: Homo sapiens

Quick links

Citations

11 review citations

Recent progress in the battle between oncolytic viruses and tumours.
Parato et al. (2005)
10 more

3 mentions without citation

A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71.
Wang et al. (2012)
2 more