› 1nlm

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF MURG:GLCNAC COMPLEX

Released:
DOI: 10.2210/pdb1nlm/pdb
PDB entry 1nlm coloured by chain, front view.
PDB entry 1nlm coloured by chain, side view.
PDB entry 1nlm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the MurG:UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases.
Hu Y, Chen L, Ha S, Gross B, Falcone B, Walker D, Mokhtarzadeh M, Walker S
Proc Natl Acad Sci U S A 100 845-9 (2003)
PMID: 12538870

Function and Biology Details

Reaction catalysed: 
UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 39.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17443 (Residues: 2-355; Coverage: 100%)
Gene names: JW0088, b0090, murG
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand UD1 2 x UD1
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 66.703Å b: 83.651Å c: 146.245Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.283
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
30 more

22 mentions without citation

The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic Targets.
Liu et al. (2016)
21 more