› 1nl2

X-ray diffraction
2.3Å resolution

BOVINE PROTHROMBIN FRAGMENT 1 IN COMPLEX WITH CALCIUM AND LYSOPHOSPHOTIDYLSERINE

Released:
DOI: 10.2210/pdb1nl2/pdb
PDB entry 1nl2 coloured by chain, front view.
PDB entry 1nl2 coloured by chain, side view.
PDB entry 1nl2 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural basis of membrane binding by Gla domains of vitamin K-dependent proteins.
Huang M, Rigby AC, Morelli X, Grant MA, Huang G, Furie B, Seaton B, Furie BC
Nat Struct Biol 10 751-6 (2003)
PMID: 12923575

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Activation peptide fragment 1 Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.86 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00735 (Residues: 44-189; Coverage: 24%)
Gene name: F2
Sequence domains:
Structure domains: Plasminogen Kringle 4

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 7 x CA
Ligand CL 3 x CL
Ligand LPS 1 x LPS
1 modified residue:
Ligand CGU 10 x CGU

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 38.894Å b: 53.217Å c: 128.959Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.274

Quick links

Citations

52 review citations

Membrane recognition by phospholipid-binding domains.
Lemmon MA. (2008)
51 more

7 mentions without citation

Phosphatidylserine receptors: enhancers of enveloped virus entry and infection.
Moller-Tank et al. (2014)
6 more