› 1ngp

X-ray diffraction
2.4Å resolution

N1G9 (IGG1-LAMBDA) FAB FRAGMENT COMPLEXED WITH (4-HYDROXY-3-NITROPHENYL) ACETATE

Released:
DOI: 10.2210/pdb1ngp/pdb
PDB entry 1ngp coloured by chain, front view.
PDB entry 1ngp coloured by chain, side view.
PDB entry 1ngp coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Three-dimensional structures of the Fab fragment of murine N1G9 antibody from the primary immune response and of its complex with (4-hydroxy-3-nitrophenyl)acetate.
Mizutani R, Miura K, Nakayama T, Shimada I, Arata Y, Satow Y
J Mol Biol 254 208-22 (1995)
PMID: 7490744

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207868 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.13 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: G0YP42 (Residues: 20-234; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
N1G9 (IGG1-LAMBDA) Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 24.08 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand NPA 1 x NPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY
Spacegroup: P41212
Unit cell:
a: 110.691Å b: 110.691Å c: 98.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.25

Quick links

Citations

4 review citations

Somatic hypermutation in mouse lambda chains.
Azuma T. (1998)
3 more

5 mentions without citation

Toward prediction of functional protein pockets using blind docking and pocket search algorithms.
Hetényi et al. (2011)
4 more