› 1n3n

X-ray diffraction
3Å resolution

Crystal structure of a mycobacterial hsp60 epitope with the murine class I MHC molecule H-2Db

Released:
DOI: 10.2210/pdb1n3n/pdb
PDB entry 1n3n coloured by chain, front view.
PDB entry 1n3n coloured by chain, side view.
PDB entry 1n3n coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural analysis of mycobacterial and murine hsp60 epitopes in complex with the class I MHC molecule H-2Db.
Ciatto C, Capitani G, Tissot AC, Pecorari F, Plückthun A, Grütter MG
FEBS Lett 543 11-5 (2003)
PMID: 12753896

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-134999 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 280 amino acids
Theoretical weight: 32.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 25-304; Coverage: 83%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
mycobacterial hsp60 decameric epitope Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 10 amino acids
Theoretical weight: 945 Da

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: C2
Unit cell:
a: 129.276Å b: 122.995Å c: 150.833Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.246 0.246 0.307
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Structural allele-specific patterns adopted by epitopes in the MHC-I cleft and reconstruction of MHC:peptide complexes to cross-reactivity assessment.
Antunes et al. (2010)