› 1n1x

X-ray diffraction
1.45Å resolution

Crystal Structure Analysis of the monomeric [S-carboxyamidomethyl-Cys31, S-carboxyamidomethyl-Cys32] Bovine seminal ribonuclease

Released:
DOI: 10.2210/pdb1n1x/pdb
PDB entry 1n1x coloured by chain, front view.
PDB entry 1n1x coloured by chain, side view.
PDB entry 1n1x coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The unswapped chain of bovine seminal ribonuclease: Crystal structure of the free and liganded monomeric derivative.
Sica F, Di Fiore A, Zagari A, Mazzarella L
Proteins 52 263-71 (2003)
PMID: 12833549

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seminal ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.75 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00669 (Residues: 27-150; Coverage: 100%)
Gene name: SRN
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand YCM 2 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 70.39Å b: 28.73Å c: 67.41Å
α: 90° β: 119° γ: 90°
R-values:
R R work R free
0.207 0.205 0.258

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Citations

1 review citation

Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.
Gotte et al. (2019)
10 other articles

1 mention without citation

The activation effect of hainantoxin-I, a peptide toxin from the Chinese spider, Ornithoctonus hainana, on intermediate-conductance Ca2+-activated K+ channels.
Huang et al. (2014)