› 1mn4

X-ray diffraction
2.2Å resolution

Structure of Ndt80 (Residues 59-340) DNA-binding domain core

Released:
DOI: 10.2210/pdb1mn4/pdb
PDB entry 1mn4 coloured by chain, front view.
PDB entry 1mn4 coloured by chain, side view.
PDB entry 1mn4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the sporulation-specific transcription factor Ndt80 bound to DNA.
Lamoureux JS, Stuart D, Tsang R, Wu C, Glover JN
EMBO J 21 5721-32 (2002)
PMID: 12411490

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Meiosis-specific transcription factor NDT80 Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P38830 (Residues: 59-340; Coverage: 45%)
Gene names: DAS1, NDT80, YHR124W
Sequence domains: NDT80 / PhoG like DNA-binding family
Structure domains: NDT80 DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P212121
Unit cell:
a: 35.88Å b: 41.93Å c: 164.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.268
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Protein-DNA recognition patterns and predictions.
Sarai et al. (2005)
2 more

3 mentions without citation

Structure of the sporulation-specific transcription factor Ndt80 bound to DNA.
Lamoureux et al. (2002)
2 more