› 1mih

X-ray diffraction
2.7Å resolution

A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY

Released:
DOI: 10.2210/pdb1mih/pdb
PDB entry 1mih coloured by chain, front view.
PDB entry 1mih coloured by chain, side view.
PDB entry 1mih coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
CheZ-mediated dephosphorylation of the Escherichia coli chemotaxis response regulator CheY: role for CheY glutamate 89.
Silversmith RE, Guanga GP, Betts L, Chu C, Zhao R, Bourret RB
J Bacteriol 185 1495-502 (2003)
PMID: 12591865

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chemotaxis protein CheY Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE67 (Residues: 1-129; Coverage: 100%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand SO4 3 x SO4
Ligand BEF 2 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 53.504Å b: 53.615Å c: 160.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.226 0.277
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial chemotaxis: a new player in response regulator dephosphorylation.
Parkinson JS. (2003)
16 other articles

1 mention without citation

Nonconserved active site residues modulate CheY autophosphorylation kinetics and phosphodonor preference.
Thomas et al. (2013)