› 1mi4

X-ray diffraction
1.7Å resolution

Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate

Released:
DOI: 10.2210/pdb1mi4/pdb
PDB entry 1mi4 coloured by chain, front view.
PDB entry 1mi4 coloured by chain, side view.
PDB entry 1mi4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli.
Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schönbrunn E
Planta 216 129-35 (2002)
PMID: 12430021

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoshikimate 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 46.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6D3 (Residues: 1-427; Coverage: 100%)
Gene names: JW0891, aroA, b0908
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:
Ligand S3P 1 x S3P
Ligand FMT 14 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 57.76Å b: 85.09Å c: 87.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.157 0.196
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Evolution in action: plants resistant to herbicides.
Powles et al. (2010)
3 more

1 mention without citation

Deciphering the structure of Arabidopsis thaliana 5-enol-pyruvyl-shikimate-3-phosphate synthase: An essential step toward the discovery of novel inhibitors to supersede glyphosate.
Ruszkowski et al. (2022)