› 1m7u

X-ray diffraction
2.8Å resolution

Crystal structure of a novel DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast

Released:
DOI: 10.2210/pdb1m7u/pdb
PDB entry 1m7u coloured by chain, front view.
PDB entry 1m7u coloured by chain, side view.
PDB entry 1m7u coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast.
Montano SP, Coté ML, Fingerman I, Pierce M, Vershon AK, Georgiadis MM
Proc Natl Acad Sci U S A 99 14041-6 (2002)
PMID: 12384578

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Meiosis-specific transcription factor NDT80 Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 31.31 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38830 (Residues: 59-330; Coverage: 43%)
Gene names: DAS1, NDT80, YHR124W
Sequence domains: NDT80 / PhoG like DNA-binding family
Structure domains: NDT80 DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 63.96Å b: 63.96Å c: 285.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.29
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Two-tier transcriptional control of oligodendrocyte differentiation.
Li et al. (2009)
3 more

1 mention without citation

Characterization of critical interactions between Ndt80 and MSE DNA defining a novel family of Ig-fold transcription factors.
Fingerman et al. (2004)