› 1m72

X-ray diffraction
2.3Å resolution

Crystal Structure of Caspase-1 from Spodoptera frugiperda

Released:
DOI: 10.2210/pdb1m72/pdb
PDB entry 1m72 coloured by chain, front view.
PDB entry 1m72 coloured by chain, side view.
PDB entry 1m72 coloured by chain, top view.
Source organism: Spodoptera frugiperda
Primary publication:
Crystal structure of an invertebrate caspase.
Forsyth CM, Lemongello D, LaCount DJ, Friesen PD, Fisher AJ
J. Biol. Chem. 279 7001-8 (2004)
PMID: 14645217

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161021 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Caspase-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 272 amino acids
Theoretical weight: 30.76 KDa
Source organism: Spodoptera frugiperda
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P89116 (Residues: 29-299; Coverage: 91%)
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Ace-Asp-Glu-Val-Asp-chloromethylketone Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 6 amino acids
Theoretical weight: 535 Da
Source organism: Spodoptera frugiperda
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3221
Unit cell:
a: 151.73Å b: 151.73Å c: 79.46Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.183 0.232
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

Molecular mechanisms of caspase regulation during apoptosis.
Riedl et al. (2004)
1 more

3 mentions without citation

Computational protein design: validation and possible relevance as a tool for homology searching and fold recognition.
Schmidt Am Busch et al. (2010)
2 more