1m6x

X-ray diffraction
2.8Å resolution

Flpe-Holliday Junction Complex

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural plasticity of the Flp-Holliday junction complex.
J Mol Biol 326 425-34 (2003)
PMID: 12559911

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-114172 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
Site-specific recombinase Flp Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 48.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03870 (Residues: 1-423; Coverage: 100%)
Gene names: FLP1, R0010W
Sequence domains:
Structure domains:
Site-specific recombinase Flp Chains: C, D
Molecule details ›
Chains: C, D
Length: 423 amino acids
Theoretical weight: 48.75 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P03870 (Residues: 1-423; Coverage: 100%)
Gene names: FLP1, R0010W
Sequence domains:
Structure domains:
Symmetrized FRT site Chains: E, F
Molecule details ›
Chains: E, F
Length: 13 nucleotides
Theoretical weight: 3.92 KDa
Symmetrized FRT site Chains: I, J
Molecule details ›
Chains: I, J
Length: 20 nucleotides
Theoretical weight: 6.17 KDa
Symmetrized FRT site Chains: G, H
Molecule details ›
Chains: G, H
Length: 33 nucleotides
Theoretical weight: 10.13 KDa

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 79.779Å b: 116.511Å c: 142.425Å
α: 90° β: 97.26° γ: 90°
R-values:
R R work R free
0.239 0.239 0.281
Expression system: Escherichia coli