› 1lq9

X-ray diffraction
1.3Å resolution

Crystal Structure of a Monooxygenase from the Gene ActVA-Orf6 of Streptomyces coelicolor Strain A3(2)

Released:
DOI: 10.2210/pdb1lq9/pdb
PDB entry 1lq9 coloured by chain, front view.
PDB entry 1lq9 coloured by chain, side view.
PDB entry 1lq9 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.
Sciara G, Kendrew SG, Miele AE, Marsh NG, Federici L, Malatesta F, Schimperna G, Savino C, Vallone B
EMBO J 22 205-15 (2003)
PMID: 12514126

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABM domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 112 amino acids
Theoretical weight: 11.98 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53908 (Residues: 2-113; Coverage: 99%)
Gene name: actVA 6
Sequence domains: Antibiotic biosynthesis monooxygenase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 47.028Å b: 60.168Å c: 71.379Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.142 0.168
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Flavoprotein monooxygenases, a diverse class of oxidative biocatalysts.
van Berkel et al. (2006)
17 more

11 mentions without citation

High-throughput crystallography for structural genomics.
Joachimiak A. (2009)
10 more