› 1lq1

X-ray diffraction
2.3Å resolution

DNA Complexed Structure of the Key Transcription Factor Initiating Development in Sporulation Bacteria

Released:
DOI: 10.2210/pdb1lq1/pdb
PDB entry 1lq1 coloured by chain, front view.
PDB entry 1lq1 coloured by chain, side view.
PDB entry 1lq1 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
DNA complexed structure of the key transcription factor initiating development in sporulating bacteria.
Zhao H, Msadek T, Zapf J, Hoch JA, Varughese KI
Structure 10 1041-50 (2002)
PMID: 12176382

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114146 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Stage 0 sporulation protein A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 120 amino acids
Theoretical weight: 13.4 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06534 (Residues: 148-267; Coverage: 45%)
Gene names: BSU24220, spo0A, spo0C, spo0G
Sequence domains: Sporulation initiation factor Spo0A C terminal
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*TP*TP*CP*GP*TP*GP*TP*CP*GP*AP*AP*TP*TP*TP*TP*G)-3' Chains: E, G
Molecule details ›
Chains: E, G
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
5'-D(*AP*CP*AP*AP*AP*AP*TP*TP*CP*GP*AP*CP*AP*CP*GP*A)-3' Chains: F, H
Molecule details ›
Chains: F, H
Length: 16 nucleotides
Theoretical weight: 4.88 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 50.917Å b: 83.301Å c: 91.241Å
α: 90° β: 94.18° γ: 90°
R-values:
R R work R free
0.23 0.217 0.258
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
6 more

9 mentions without citation

Identification of sensory and signal-transducing domains in two-component signaling systems.
Galperin et al. (2007)
8 more