› 1lpf

X-ray diffraction
2.8Å resolution

THREE-DIMENSIONAL STRUCTURE OF LIPOAMIDE DEHYDROGENASE FROM PSEUDOMONAS FLUORESCENS AT 2.8 ANGSTROMS RESOLUTION. ANALYSIS OF REDOX AND THERMOSTABILITY PROPERTIES

Released:
DOI: 10.2210/pdb1lpf/pdb
PDB entry 1lpf coloured by chain, front view.
PDB entry 1lpf coloured by chain, side view.
PDB entry 1lpf coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Three-dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8 A resolution. Analysis of redox and thermostability properties.
Mattevi A, Obmolova G, Kalk KH, van Berkel WJ, Hol WG
J Mol Biol 230 1200-15 (1993)
PMID: 8487301

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyl dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 477 amino acids
Theoretical weight: 50.08 KDa
Source organism: Pseudomonas fluorescens
Expression system: Not provided
UniProt:
  • Canonical: P14218 (Residues: 2-478; Coverage: 100%)
Gene name: lpd
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 113.6Å b: 66.4Å c: 164.3Å
α: 90° β: 99.2° γ: 90°
R-values:
R R work R free
0.192 0.192 not available
Expression system: Not provided

Quick links

Citations

4 review citations

The pyruvate dehydrogenase multi-enzyme complex from Gram-negative bacteria.
de Kok et al. (1998)
3 more

3 mentions without citation

The udhA gene of Escherichia coli encodes a soluble pyridine nucleotide transhydrogenase.
Boonstra et al. (1999)
2 more