› 1lmh

X-ray diffraction
1.9Å resolution

Crystal Structure of S. aureus peptide deformylase

Released:
DOI: 10.2210/pdb1lmh/pdb
PDB entry 1lmh coloured by chain, front view.
PDB entry 1lmh coloured by chain, side view.
PDB entry 1lmh coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structure of type II peptide deformylase from Staphylococcus aureus.
Baldwin ET, Harris MS, Yem AW, Wolfe CL, Vosters AF, Curry KA, Murray RW, Bock JH, Marshall VP, Cialdella JI, Merchant MH, Choi G, Deibel MR
J Biol Chem 277 31163-71 (2002)
PMID: 12048187

Function and Biology Details

Reaction catalysed: 
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide deformylase Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.98 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68826 (Residues: 1-183; Coverage: 100%)
Gene names: def, def1, pdf1
Sequence domains: Polypeptide deformylase
Structure domains: Peptide deformylase

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2221
Unit cell:
a: 94.131Å b: 121.873Å c: 47.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.212
Expression system: Escherichia coli

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Citations

4 review citations

Structural aspects of the metzincin clan of metalloendopeptidases.
Gomis-Rüth FX. (2003)
3 more