› 1llu

X-ray diffraction
2.3Å resolution

THE TERNARY COMPLEX OF PSEUDOMONAS AERUGINOSA ALCOHOL DEHYDROGENASE WITH ITS COENZYME AND WEAK SUBSTRATE

Released:
DOI: 10.2210/pdb1llu/pdb
PDB entry 1llu coloured by chain, front view.
PDB entry 1llu coloured by chain, side view.
PDB entry 1llu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
The ternary complex of Pseudomonas aeruginosa alcohol dehydrogenase with NADH and ethylene glycol.
Levin I, Meiri G, Peretz M, Burstein Y, Frolow F
Protein Sci 13 1547-56 (2004)
PMID: 15152088

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190923 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alcohol dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 342 amino acids
Theoretical weight: 35.92 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HTD9 (Residues: 1-342; Coverage: 100%)
Gene names: PA5427, adhA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
2 bound ligands:
Ligand ZN 16 x ZN
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 74.171Å b: 86.328Å c: 125.698Å
α: 79.34° β: 78.66° γ: 71.58°
R-values:
R R work R free
0.229 0.229 0.246
Expression system: Escherichia coli

Quick links

Citations

22 citation in other articles

Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales.
Daly et al. (2016)
21 more

7 mentions without citation

Yeast alcohol dehydrogenase structure and catalysis.
Raj et al. (2014)
6 more