› 1ljm

X-ray diffraction
2.5Å resolution

DNA recognition is mediated by conformational transition and by DNA bending

Released:
DOI: 10.2210/pdb1ljm/pdb
PDB entry 1ljm coloured by chain, front view.
PDB entry 1ljm coloured by chain, side view.
PDB entry 1ljm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA recognition by the RUNX1 transcription factor is mediated by an allosteric transition in the RUNT domain and by DNA bending.
Bartfeld D, Shimon L, Couture GC, Rabinovich D, Frolow F, Levanon D, Groner Y, Shakked Z
Structure 10 1395-407 (2002)
PMID: 12377125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-169533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Runt-related transcription factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01196 (Residues: 51-181; Coverage: 29%)
Gene names: AML1, CBFA2, RUNX1
Sequence domains: Runt domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: R32
Unit cell:
a: 121.23Å b: 121.23Å c: 186.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.249
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
8 more

5 mentions without citation

MuPIT interactive: webserver for mapping variant positions to annotated, interactive 3D structures.
Niknafs et al. (2013)
4 more