› 1lj2

X-ray diffraction
2.38Å resolution

Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization

Released:
DOI: 10.2210/pdb1lj2/pdb
PDB entry 1lj2 coloured by chain, front view.
PDB entry 1lj2 coloured by chain, side view.
PDB entry 1lj2 coloured by chain, top view.
Source organisms:
Primary publication:
Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization.
Groft CM, Burley SK
Mol Cell 9 1273-83 (2002)
PMID: 12086624

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137115 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Non-structural protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.84 KDa
Source organism: Simian rotavirus A/SA11
Expression system: Escherichia coli
UniProt:
  • Canonical: P03536 (Residues: 206-315; Coverage: 35%)
Structure domains: Nonstructural RNA-binding protein
Eukaryotic translation initiation factor 4 gamma 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 28 amino acids
Theoretical weight: 3.2 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q04637 (Residues: 172-199; Coverage: 2%)
Gene names: EIF4F, EIF4G, EIF4G1, EIF4GI

Ligands and Environments

1 bound ligand:
Ligand AU 3 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 59.69Å b: 74.04Å c: 77.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.272
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

28 review citations

Poly(A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression.
Mangus et al. (2003)
27 more

3 mentions without citation

Rotavirus non-structural proteins: structure and function.
Hu et al. (2012)
2 more