1ldc

X-ray diffraction
2.9Å resolution

X-RAY STRUCTURE OF TWO COMPLEXES OF THE Y143F FLAVOCYTOCHROME B2 MUTANT CRYSTALLIZED IN THE PRESENCE OF LACTATE OR PHENYL-LACTATE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-132308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase (cytochrome) Chains: A, B
Molecule details ›
Chains: A, B
Length: 511 amino acids
Theoretical weight: 56.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00175 (Residues: 81-591; Coverage: 87%)
Gene names: CYB2, YM9958.08C, YML054C
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN

Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 164.5Å b: 164.5Å c: 114Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 not available
Expression system: Escherichia coli