› 1kv9

X-ray diffraction
1.9Å resolution

Structure at 1.9 A Resolution of a Quinohemoprotein Alcohol Dehydrogenase from Pseudomonas putida HK5

Released:
DOI: 10.2210/pdb1kv9/pdb
PDB entry 1kv9 coloured by chain, front view.
PDB entry 1kv9 coloured by chain, side view.
PDB entry 1kv9 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Structure at 1.9 A resolution of a quinohemoprotein alcohol dehydrogenase from Pseudomonas putida HK5.
Chen ZW, Matsushita K, Yamashita T, Fujii TA, Toyama H, Adachi O, Bellamy HD, Mathews FS
Structure 10 837-49 (2002)
PMID: 12057198

Function and Biology Details

Reaction catalysed: 
A primary alcohol + azurin = an aldehyde + reduced azurin
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinohemoprotein alcohol dehydrogenase ADH IIB Chain: A
Molecule details ›
Chain: A
Length: 668 amino acids
Theoretical weight: 72.71 KDa
Source organism: Pseudomonas putida
UniProt:
  • Canonical: Q8GR64 (Residues: 23-690; Coverage: 100%)
Gene name: qbdA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEC 1 x HEC
5 bound ligands:
Ligand CA 1 x CA
Ligand PQQ 1 x PQQ
Ligand EPE 1 x EPE
Ligand ACN 1 x ACN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 54.807Å b: 57.437Å c: 67.523Å
α: 89.65° β: 69.34° γ: 68.39°
R-values:
R R work R free
0.154 0.154 0.189

Quick links

Citations

8 review citations

PQQ-dependent methanol dehydrogenases: rare-earth elements make a difference.
Keltjens et al. (2014)
7 more

6 mentions without citation

Protein secretion and outer membrane assembly in Alphaproteobacteria.
Gatsos et al. (2008)
5 more