› 1ks4

X-ray diffraction
2.5Å resolution

The structure of Aspergillus niger endoglucanase-palladium complex

Released:
DOI: 10.2210/pdb1ks4/pdb
PDB entry 1ks4 coloured by chain, front view.
PDB entry 1ks4 coloured by chain, side view.
PDB entry 1ks4 coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Determination of the structure of an endoglucanase from Aspergillus niger and its mode of inhibition by palladium chloride.
Khademi S, Zhang D, Swanson SM, Wartenberg A, Witte K, Meyer EF
Acta Crystallogr D Biol Crystallogr 58 660-7 (2002)
PMID: 11914491

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 24.28 KDa
Source organism: Aspergillus niger
UniProt:
  • Canonical: O74705 (Residues: 17-239; Coverage: 100%)
Gene names: AnigIFM63604_000202, CAN33_0026800, eglA
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand PD 3 x PD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I422
Unit cell:
a: 131.088Å b: 131.088Å c: 71.985Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.214 0.246

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Citations

1 review citation

Genomics review of holocellulose deconstruction by aspergilli.
Segato et al. (2014)
9 other articles

6 mentions without citation

Non-homologous isofunctional enzymes: a systematic analysis of alternative solutions in enzyme evolution.
Omelchenko et al. (2010)
5 more