› 1kk4

X-ray diffraction
2.7Å resolution

Crystal Structure of Vat(D) in Complex with Acetyl-CoA

Released:
DOI: 10.2210/pdb1kk4/pdb
PDB entry 1kk4 coloured by chain, front view.
PDB entry 1kk4 coloured by chain, side view.
PDB entry 1kk4 coloured by chain, top view.
Source organism: Enterococcus faecium
Primary publication:
Crystal structure of Vat(D): an acetyltransferase that inactivates streptogramin group A antibiotics.
Sugantino M, Roderick SL
Biochemistry 41 2209-16 (2002)
PMID: 11841212

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptogramin A acetyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 209 amino acids
Theoretical weight: 23.68 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50870 (Residues: 1-209; Coverage: 100%)
Gene names: satA, vatD
Sequence domains: Bacterial transferase hexapeptide (six repeats)
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

1 bound ligand:
Ligand ACO 6 x ACO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: F222
Unit cell:
a: 186.3Å b: 186.3Å c: 186.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The antibiotic resistome: the nexus of chemical and genetic diversity.
Wright GD. (2007)
9 more

1 mention without citation

Structural and functional characterization of three Type B and C chloramphenicol acetyltransferases from Vibrio species.
Alcala et al. (2020)