› 1kil

X-ray diffraction
2.3Å resolution

Three-dimensional structure of the complexin/SNARE complex

Released:
DOI: 10.2210/pdb1kil/pdb
PDB entry 1kil coloured by chain, front view.
PDB entry 1kil coloured by chain, side view.
PDB entry 1kil coloured by chain, top view.
Source organisms:
Primary publication:
Three-dimensional structure of the complexin/SNARE complex.
Chen X, Tomchick DR, Kovrigin E, Araç D, Machius M, Südhof TC, Rizo J
Neuron 33 397-409 (2002)
PMID: 11832227

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-152473 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-associated membrane protein 2 Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.66 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63045 (Residues: 28-92; Coverage: 56%)
Gene names: Syb2, Vamp2
Sequence domains: Synaptobrevin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 62 amino acids
Theoretical weight: 7.19 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32851 (Residues: 190-250; Coverage: 21%)
Gene names: Sap, Stx1a
Sequence domains: SNARE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: C
Molecule details ›
Chain: C
Length: 74 amino acids
Theoretical weight: 8.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60880 (Residues: 11-81; Coverage: 35%)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: D
Molecule details ›
Chain: D
Length: 66 amino acids
Theoretical weight: 7.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60880 (Residues: 139-203; Coverage: 32%)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Complexin-1 Chain: E
Molecule details ›
Chain: E
Length: 49 amino acids
Theoretical weight: 5.77 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63041 (Residues: 26-72; Coverage: 35%)
Gene name: Cplx1
Sequence domains: Synaphin protein
Structure domains: Single Helix bin

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 40.489Å b: 60.425Å c: 159.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.287 0.245 0.305
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

60 review citations

SNAREs--engines for membrane fusion.
Jahn et al. (2006)
59 more

37 mentions without citation

Mechanism of neurotransmitter release coming into focus.
Rizo J. (2018)
36 more