› 1ka1

X-ray diffraction
1.3Å resolution

The PAPase Hal2p complexed with calcium and magnesium ions and reaction substrate: PAP

Released:
DOI: 10.2210/pdb1ka1/pdb
PDB entry 1ka1 coloured by chain, front view.
PDB entry 1ka1 coloured by chain, side view.
PDB entry 1ka1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases.
Patel S, Martínez-Ripoll M, Blundell TL, Albert A
J Mol Biol 320 1087-94 (2002)
PMID: 12126627

Function and Biology Details

Reaction catalysed: 
Adenosine 3',5'-bisphosphate + H(2)O = adenosine 5'-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3'(2'),5'-bisphosphate nucleotidase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 39.2 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32179 (Residues: 1-357; Coverage: 100%)
Gene names: HAL2, MET22, YOL064C
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand MG 1 x MG
Ligand A3P 1 x A3P
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 54.875Å b: 44.968Å c: 72.11Å
α: 90° β: 110.72° γ: 90°
R-values:
R R work R free
0.134 0.133 0.169
Expression system: Saccharomyces cerevisiae

Quick links

Citations

5 review citations

Drugs, their targets and the nature and number of drug targets.
Imming et al. (2006)
4 more

5 mentions without citation

Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase.
Chan et al. (2016)
4 more