› 1k78

X-ray diffraction
2.25Å resolution

Pax5(1-149)+Ets-1(331-440)+DNA

Released:
DOI: 10.2210/pdb1k78/pdb
PDB entry 1k78 coloured by chain, front view.
PDB entry 1k78 coloured by chain, side view.
PDB entry 1k78 coloured by chain, top view.
Source organisms:
Primary publication:
Structural studies of Ets-1/Pax5 complex formation on DNA.
Garvie CW, Hagman J, Wolberger C
Mol Cell 8 1267-76 (2001)
PMID: 11779502

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero pentamer
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114055 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Paired box protein Pax-5 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 149 amino acids
Theoretical weight: 16.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02548 (Residues: 1-149; Coverage: 38%)
Gene name: PAX5
Sequence domains: 'Paired box' domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Protein C-ets-1 Chains: B, F
Molecule details ›
Chains: B, F
Length: 110 amino acids
Theoretical weight: 12.99 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27577 (Residues: 331-440; Coverage: 25%)
Gene names: Ets-1, Ets1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Pax5/Ets Binding Site on the mb-1 promoter Chains: C, G
Molecule details ›
Chains: C, G
Length: 27 nucleotides
Theoretical weight: 8.37 KDa
Pax5/Ets Binding Site on the mb-1 promoter Chains: D, H
Molecule details ›
Chains: D, H
Length: 27 nucleotides
Theoretical weight: 8.23 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D, NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 77.84Å b: 89.75Å c: 170.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.227 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Pax5: the guardian of B cell identity and function.
Cobaleda et al. (2007)
24 more

17 mentions without citation

Recent advances in the structural molecular biology of Ets transcription factors: interactions, interfaces and inhibition.
Cooper et al. (2014)
16 more