› 1k4q

X-ray diffraction
1.9Å resolution

Human Glutathione Reductase Inactivated by Peroxynitrite

Released:
DOI: 10.2210/pdb1k4q/pdb
PDB entry 1k4q coloured by chain, front view.
PDB entry 1k4q coloured by chain, side view.
PDB entry 1k4q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the antioxidant enzyme glutathione reductase inactivated by peroxynitrite.
Savvides SN, Scheiwein M, Bohme CC, Arteel GE, Karplus PA, Becker K, Schirmer RH
J Biol Chem 277 2779-84 (2002)
PMID: 11705998

Function and Biology Details

Reaction catalysed: 
2 glutathione + NADP(+) = glutathione disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione reductase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 50.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00390 (Residues: 62-522; Coverage: 88%)
Gene names: GLUR, GRD1, GSR
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
1 modified residue:
Ligand NIY 2 x NIY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 119.75Å b: 63.65Å c: 84.71Å
α: 90° β: 58.42° γ: 90°
R-values:
R R work R free
0.166 0.166 0.204
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Peroxynitrite: biochemistry, pathophysiology and development of therapeutics.
Szabó et al. (2007)
17 more

3 mentions without citation

Thioredoxin reductase and its inhibitors.
Saccoccia et al. (2014)
2 more