› 1k1x

X-ray diffraction
2.4Å resolution

Crystal structure of 4-alpha-glucanotransferase from thermococcus litoralis

Released:
DOI: 10.2210/pdb1k1x/pdb
PDB entry 1k1x coloured by chain, front view.
PDB entry 1k1x coloured by chain, side view.
PDB entry 1k1x coloured by chain, top view.
Source organism: Thermococcus litoralis
Primary publication:
Crystal structures of 4-alpha-glucanotransferase from Thermococcus litoralis and its complex with an inhibitor.
Imamura H, Fushinobu S, Yamamoto M, Kumasaka T, Jeon BS, Wakagi T, Matsuzawa H
J Biol Chem 278 19378-86 (2003)
PMID: 12618437

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-128449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-alpha-glucanotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 659 amino acids
Theoretical weight: 77.99 KDa
Source organism: Thermococcus litoralis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32462 (Residues: 1-659; Coverage: 100%)
Gene names: OCC_10078, jgt
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX
Spacegroup: P21212
Unit cell:
a: 137.682Å b: 161.494Å c: 70.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
2 more

4 mentions without citation

Crystal structure of exotype alginate lyase Atu3025 from Agrobacterium tumefaciens.
Ochiai et al. (2010)
3 more