› 1jzg

X-ray diffraction
1.4Å resolution

Pseudomonas aeruginosa Reduced Azurin (Cu1+) Ru(tpy)(phen)(His83)

Released:
DOI: 10.2210/pdb1jzg/pdb
PDB entry 1jzg coloured by chain, front view.
PDB entry 1jzg coloured by chain, side view.
PDB entry 1jzg coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Electron tunneling in single crystals of Pseudomonas aeruginosa azurins.
Crane BR, Di Bilio AJ, Winkler JR, Gray HB
J Am Chem Soc 123 11623-31 (2001)
PMID: 11716717

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.96 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU1 1 x CU1
Ligand RTB 1 x RTB
Ligand IMF 1 x IMF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I222
Unit cell:
a: 52.747Å b: 61.608Å c: 76.187Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Copper in diseases and treatments, and copper-based anticancer strategies.
Tisato et al. (2010)
11 more

5 mentions without citation

Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs.
Bernardes et al. (2018)
4 more