› 1jx1

X-ray diffraction
2.3Å resolution

Chalcone Isomerase--T48A mutant

Released:
DOI: 10.2210/pdb1jx1/pdb
PDB entry 1jx1 coloured by chain, front view.
PDB entry 1jx1 coloured by chain, side view.
PDB entry 1jx1 coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Role of hydrogen bonds in the reaction mechanism of chalcone isomerase.
Jez JM, Bowman ME, Noel JP
Biochemistry 41 5168-76 (2002)
PMID: 11955065

Function and Biology Details

Reaction catalysed: 
A chalcone = a flavanone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone--flavanone isomerase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28012 (Residues: 1-222; Coverage: 100%)
Gene names: CHI-1, CHI1
Sequence domains: Chalcone-flavanone isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand DFV 1 x DFV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P65
Unit cell:
a: 90.94Å b: 90.94Å c: 349.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Metabolic channeling in plants.
Winkel BS. (2004)
6 more

3 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
2 more