› 1jki

X-ray diffraction
2.2Å resolution

myo-Inositol-1-phosphate Synthase Complexed with an Inhibitor, 2-deoxy-glucitol-6-phosphate

Released:
DOI: 10.2210/pdb1jki/pdb
PDB entry 1jki coloured by chain, front view.
PDB entry 1jki coloured by chain, side view.
PDB entry 1jki coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure and mechanism of 1-L-myo-inositol- 1-phosphate synthase.
Stein AJ, Geiger JH
J Biol Chem 277 9484-91 (2002)
PMID: 11779862

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146022 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-3-phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 59.71 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11986 (Residues: 1-533; Coverage: 100%)
Gene names: INO1, J0610, YJL153C
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
2 bound ligands:
Ligand NH4 2 x NH4
Ligand DG6 2 x DG6
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 152.728Å b: 98.309Å c: 121.855Å
α: 90° β: 126.093° γ: 90°
R-values:
R R work R free
0.285 0.208 0.28
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biosynthesis of aminocyclitol-aminoglycoside antibiotics and related compounds.
Flatt et al. (2007)
2 more

4 mentions without citation

The Genome Sequence of the Wild Tomato Solanum pimpinellifolium Provides Insights Into Salinity Tolerance.
Razali et al. (2018)
3 more