› 1jbv

X-ray diffraction
1.95Å resolution

FPGS-AMPPCP complex

Released:
DOI: 10.2210/pdb1jbv/pdb
PDB entry 1jbv coloured by chain, front view.
PDB entry 1jbv coloured by chain, side view.
PDB entry 1jbv coloured by chain, top view.
Source organism: Lacticaseibacillus casei
Primary publication:
Folate-binding triggers the activation of folylpolyglutamate synthetase.
Sun X, Cross JA, Bognar AL, Baker EN, Smith CA
J Mol Biol 310 1067-78 (2001)
PMID: 11501996

Function and Biology Details

Reaction catalysed: 
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Folylpolyglutamate synthase Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 46.7 KDa
Source organism: Lacticaseibacillus casei
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P15925 (Residues: 1-428; Coverage: 100%)
Gene name: fpgS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ACP 1 x ACP
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 53.1Å b: 45Å c: 84.3Å
α: 90° β: 107.1° γ: 90°
R-values:
R R work R free
0.194 0.194 0.237
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
6 more

1 mention without citation

Identification of protein biochemical functions by similarity search using the molecular surface database eF-site.
Kinoshita et al. (2003)