› 1j9y

X-ray diffraction
1.85Å resolution

Crystal structure of mannanase 26A from Pseudomonas cellulosa

Released:
DOI: 10.2210/pdb1j9y/pdb
PDB entry 1j9y coloured by chain, front view.
PDB entry 1j9y coloured by chain, side view.
PDB entry 1j9y coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
Crystal structure of mannanase 26A from Pseudomonas cellulosa and analysis of residues involved in substrate binding.
Hogg D, Woo EJ, Bolam DN, McKie VA, Gilbert HJ, Pickersgill RW
J Biol Chem 276 31186-92 (2001)
PMID: 11382747

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan endo-1,4-beta-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 43.62 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P49424 (Residues: 39-423; Coverage: 97%)
Gene names: CJA_2770, man26A, manA
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P41
Unit cell:
a: 93.24Å b: 93.24Å c: 54.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.2
Expression system: Escherichia coli BL21

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Citations

10 review citations

Microbial hemicellulases.
Shallom et al. (2003)
9 more

2 mentions without citation

The Cellvibrio japonicus mannanase CjMan26C displays a unique exo-mode of action that is conferred by subtle changes to the distal region of the active site.
Cartmell et al. (2008)
1 more