› 1j8u

X-ray diffraction
1.5Å resolution

Catalytic Domain of Human Phenylalanine Hydroxylase Fe(II) in Complex with Tetrahydrobiopterin

Released:
DOI: 10.2210/pdb1j8u/pdb
PDB entry 1j8u coloured by chain, front view.
PDB entry 1j8u coloured by chain, side view.
PDB entry 1j8u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structures of the catalytic domain of human phenylalanine hydroxylase in its catalytically active Fe(II) form and binary complex with tetrahydrobiopterin.
Andersen OA, Flatmark T, Hough E
J Mol Biol 314 279-91 (2001)
PMID: 11718561

Function and Biology Details

Reaction catalysed: 
L-phenylalanine + tetrahydrobiopterin + O(2) = L-tyrosine + 4a-hydroxytetrahydrobiopterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine-4-hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 37.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00439 (Residues: 103-427; Coverage: 72%)
Gene name: PAH
Sequence domains: Biopterin-dependent aromatic amino acid hydroxylase
Structure domains: Aromatic amino acid hydroxylase

Ligands and Environments


Cofactor: Ligand H4B 1 x H4B
1 bound ligand:
Ligand FE2 1 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: C2221
Unit cell:
a: 66.441Å b: 108.676Å c: 124.418Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.203
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Mononuclear non-heme iron enzymes with the 2-His-1-carboxylate facial triad: recent developments in enzymology and modeling studies.
Bruijnincx et al. (2008)
5 more

19 mentions without citation

Mechanism of aromatic amino acid hydroxylation.
Fitzpatrick PF. (2003)
18 more