› 1j0q

Solution NMR

Solution Structure of Oxidized Bovine Microsomal Cytochrome b5 mutant V61H

Released:
DOI: 10.2210/pdb1j0q/pdb
PDB entry 1j0q coloured by chain, ensemble of 35 models, front view.
PDB entry 1j0q coloured by chain, ensemble of 35 models, side view.
PDB entry 1j0q coloured by chain, ensemble of 35 models, top view.
Source organism: Bos taurus
Primary publication:
The solution structure of the oxidized bovine microsomal cytochrome b(5) mutant V61H.
Cao C, Zhang Q, Xue LL, Ma J, Wang YH, Wu H, Huang ZX
Biochem Biophys Res Commun 307 600-9 (2003)
PMID: 12893266

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.51 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00171 (Residues: 8-89; Coverage: 61%)
Gene names: CYB5, CYB5A
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Direct electrochemical analyses of human cytochromes b5 with a mutated heme pocket showed a good correlation between their midpoint and half wave potentials.
Aono et al. (2010)